Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus
Identifieur interne : 001F64 ( Main/Exploration ); précédent : 001F63; suivant : 001F65Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus
Auteurs : M. Dutt [États-Unis] ; J. W. Grosser [États-Unis]Source :
- Plant cell, tissue and organ culture [ 0167-6857 ] ; 2009.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
An improved protocol for genetic transformation of juvenile explants of Carrizo (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.), Duncan (Citrus paradisi Macf.), Hamlin (Citrus sinensis (L.) Osbeck) and Mexican Lime (Citrus aurantifolia Swingle) cultivars using a vector containing a bifunctional egfp-nptII fusion gene is described. Several parameters were investigated to optimize genetic transformation of these four cultivars. It was determined that a short preincubation in hormone rich liquid medium and subculture of Agrobacterium for 3 h in YEP medium containing 100 μM acetosyringone were required for improvement of transformation efficiency. Co-cultivation duration as well as addition of acetosyringone to co-cultivation medium also played an important role in transformation efficiency as did OD600 value of the Agrobacterium suspension used for transformation. We regenerated numerous EGFP expressing transgenic lines from all four cultivars. Based on these results, we conclude that genetic transformation of citrus is cultivar specific and optimization of conditions for maximum transgenic production are required for each individual cultivar.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000298
- to stream PascalFrancis, to step Curation: 000705
- to stream PascalFrancis, to step Checkpoint: 000313
- to stream Main, to step Merge: 002014
- to stream Main, to step Curation: 001F64
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus</title><author><name sortKey="Dutt, M" sort="Dutt, M" uniqKey="Dutt M" first="M." last="Dutt">M. Dutt</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Horticultural Sciences Department, Citrus Research and Education Center (CREC), University of Florida-IFAS, 700 Experiment Station Road</s1><s2>Lake Alfred, FL 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Grosser, J W" sort="Grosser, J W" uniqKey="Grosser J" first="J. W." last="Grosser">J. W. Grosser</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Horticultural Sciences Department, Citrus Research and Education Center (CREC), University of Florida-IFAS, 700 Experiment Station Road</s1><s2>Lake Alfred, FL 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">09-0379508</idno><date when="2009">2009</date><idno type="stanalyst">PASCAL 09-0379508 INIST</idno><idno type="RBID">Pascal:09-0379508</idno><idno type="wicri:Area/PascalFrancis/Corpus">000298</idno><idno type="wicri:Area/PascalFrancis/Curation">000705</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000313</idno><idno type="wicri:doubleKey">0167-6857:2009:Dutt M:evaluation:of:parameters</idno><idno type="wicri:Area/Main/Merge">002014</idno><idno type="wicri:Area/Main/Curation">001F64</idno><idno type="wicri:Area/Main/Exploration">001F64</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus</title><author><name sortKey="Dutt, M" sort="Dutt, M" uniqKey="Dutt M" first="M." last="Dutt">M. Dutt</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Horticultural Sciences Department, Citrus Research and Education Center (CREC), University of Florida-IFAS, 700 Experiment Station Road</s1><s2>Lake Alfred, FL 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Grosser, J W" sort="Grosser, J W" uniqKey="Grosser J" first="J. W." last="Grosser">J. W. Grosser</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Horticultural Sciences Department, Citrus Research and Education Center (CREC), University of Florida-IFAS, 700 Experiment Station Road</s1><s2>Lake Alfred, FL 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Plant cell, tissue and organ culture</title><title level="j" type="abbreviated">Plant cell tissue organ cult.</title><idno type="ISSN">0167-6857</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant cell, tissue and organ culture</title><title level="j" type="abbreviated">Plant cell tissue organ cult.</title><idno type="ISSN">0167-6857</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Citrus</term><term>Gene</term><term>Genetic transformation</term><term>Transfection</term><term>Vegetals</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Transformation génétique</term><term>Agrobacterium tumefaciens</term><term>Transfection</term><term>Gène</term><term>Végétal</term><term>Citrus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An improved protocol for genetic transformation of juvenile explants of Carrizo (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.), Duncan (Citrus paradisi Macf.), Hamlin (Citrus sinensis (L.) Osbeck) and Mexican Lime (Citrus aurantifolia Swingle) cultivars using a vector containing a bifunctional egfp-nptII fusion gene is described. Several parameters were investigated to optimize genetic transformation of these four cultivars. It was determined that a short preincubation in hormone rich liquid medium and subculture of Agrobacterium for 3 h in YEP medium containing 100 μM acetosyringone were required for improvement of transformation efficiency. Co-cultivation duration as well as addition of acetosyringone to co-cultivation medium also played an important role in transformation efficiency as did OD<sub>600</sub> value of the Agrobacterium suspension used for transformation. We regenerated numerous EGFP expressing transgenic lines from all four cultivars. Based on these results, we conclude that genetic transformation of citrus is cultivar specific and optimization of conditions for maximum transgenic production are required for each individual cultivar.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Dutt, M" sort="Dutt, M" uniqKey="Dutt M" first="M." last="Dutt">M. Dutt</name></region><name sortKey="Grosser, J W" sort="Grosser, J W" uniqKey="Grosser J" first="J. W." last="Grosser">J. W. Grosser</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F64 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001F64 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:09-0379508
|texte= Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus
}}
| This area was generated with Dilib version V0.6.25. |  |